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radiolabeled sense (control) and antisense crna probes generated by in vitro transcription with α-[35s]-utp  (New England Nuclear Corporation)

 
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    New England Nuclear Corporation radiolabeled sense (control) and antisense crna probes generated by in vitro transcription with α-[35s]-utp
    Radiolabeled Sense (Control) And Antisense Crna Probes Generated By In Vitro Transcription With α [35s] Utp, supplied by New England Nuclear Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antisense+and+sense+crna+probes/%CE%B1+35s+datp/pm19915939-73-23-26
    Average 90 stars, based on 1 article reviews
    radiolabeled sense (control) and antisense crna probes generated by in vitro transcription with α-[35s]-utp - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Synthesized:

    Article Title: Alterations in central neuropeptide expression, release, and receptor binding in rats bred for high anxiety: critical role of vasopressin.
    Article Snippet: .. Antisense and sense cRNA probes were synthesized incorporating [35S]CTP (New England Nuclear, Boston, MA) at a specific activity of 9 108 cpm/mg probe. ..

    Activity Assay:

    Article Title: Alterations in central neuropeptide expression, release, and receptor binding in rats bred for high anxiety: critical role of vasopressin.
    Article Snippet: .. Antisense and sense cRNA probes were synthesized incorporating [35S]CTP (New England Nuclear, Boston, MA) at a specific activity of 9 108 cpm/mg probe. ..



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    In situ expression of the lncRNA MALAT1 in vivo. Mice were exposed to normoxia or hypoxia (8% O 2 ) for 24 hours. Notes: In situ hybridization was performed with <t>digoxigenin-labeled</t> antisense or sense probes followed by detection with alkaline phosphatase-labeled anti-digoxigenin antibodies and nitro-blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate staining using kidney ( A ) and testis ( C ) tissue slices. ( B ) MALAT1 positive renal proximal and distal tubules of three visual fields were counted and shown as mean values + SEM of three independent sections (* P <0.001, Student’s t -test). Abbreviations: h, hours; lncRNAs, long noncoding RNAs; MALAT1, metastasis-associated lung adenocarcinoma transcript 1; SEM, standard error of mean.
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    Image Search Results


    In situ expression of the lncRNA MALAT1 in vivo. Mice were exposed to normoxia or hypoxia (8% O 2 ) for 24 hours. Notes: In situ hybridization was performed with digoxigenin-labeled antisense or sense probes followed by detection with alkaline phosphatase-labeled anti-digoxigenin antibodies and nitro-blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate staining using kidney ( A ) and testis ( C ) tissue slices. ( B ) MALAT1 positive renal proximal and distal tubules of three visual fields were counted and shown as mean values + SEM of three independent sections (* P <0.001, Student’s t -test). Abbreviations: h, hours; lncRNAs, long noncoding RNAs; MALAT1, metastasis-associated lung adenocarcinoma transcript 1; SEM, standard error of mean.

    Journal: Hypoxia

    Article Title: Induction of long noncoding RNA MALAT1 in hypoxic mice

    doi: 10.2147/HP.S90555

    Figure Lengend Snippet: In situ expression of the lncRNA MALAT1 in vivo. Mice were exposed to normoxia or hypoxia (8% O 2 ) for 24 hours. Notes: In situ hybridization was performed with digoxigenin-labeled antisense or sense probes followed by detection with alkaline phosphatase-labeled anti-digoxigenin antibodies and nitro-blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate staining using kidney ( A ) and testis ( C ) tissue slices. ( B ) MALAT1 positive renal proximal and distal tubules of three visual fields were counted and shown as mean values + SEM of three independent sections (* P <0.001, Student’s t -test). Abbreviations: h, hours; lncRNAs, long noncoding RNAs; MALAT1, metastasis-associated lung adenocarcinoma transcript 1; SEM, standard error of mean.

    Article Snippet: Following subcloning into pBluescriptKS+, digoxigenin-labeled cRNA sense and antisense probes were synthesized using T3 and T7 RNA polymerases (ThermoScientific).

    Techniques: In Situ, Expressing, In Vivo, In Situ Hybridization, Labeling, Staining